Easiest Way to Download All Sra Samples or Multi Experiment file from NCBI SRA database
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European Nucleotide Archive is good place to start to download the raw fastq files. But it is not easy to download multiple run files from NCBI SRA database. I recently learn to download in relatively easy way. I want to download these four run together from NCBI SRA database : SRR122247,SRR122248,SRR122249, SRR122250. Format of basic url is like that
ftp://ftp-trace.ncbi.nih.gov/sra/sra-instant/reads/ByRun/sra/{SRR|ERR|DRR}/<first 6 characters of accession>/<accession>/<accession>.sra
Where
{SRR|ERR|DRR} should be either ‘SRR’, ‘ERR’, or ‘DRR’ and should match the prefix of the target .sra file
Described in details HERE.
So my final urls will look like this
ftp://ftp-trace.ncbi.nih.gov/sra/sra-instant/reads/ByRun/sra/SRR/SRR122/SRR122247/SRR122247.sra ftp://ftp-trace.ncbi.nih.gov/sra/sra-instant/reads/ByRun/sra/SRR/SRR122/SRR122248/SRR122248.sra ftp://ftp-trace.ncbi.nih.gov/sra/sra-instant/reads/ByRun/sra/SRR/SRR122/SRR122249/SRR122249.sra ftp://ftp-trace.ncbi.nih.gov/sra/sra-instant/reads/ByRun/sra/SRR/SRR122/SRR122250/SRR122250.sraNow save this urls in a text file (url_list.txt, for example). Run the wget like this
wget -i url_list.txt
run sudo apt-get install wget from terminal if you don't have wget
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